Paul Vouros

Faculty Fellow, Barnett Institute; Bradstreet Chair in Bioanalytical Chemistry and Biomedical Sciences; Professor, Dept. of Chemistry and Chemical Biology The Barnett Institute, 341 Mugar Hall Northeastern University  360 Huntington Avenue  Boston MA 02115  email: p.vouros@neu.edu  voice: 617-373-2840  fax: 617-373-2855  Vouros Research Group Page Chemistry Dept. Vouros Page

Education

1965 Ph.D., MIT

1961 B.A., Wesleyan University

Research Interests

The central focus of our research is in the area of mass spectrometry and its applications to organic analysis. Our efforts involve the investigation of both fundamental and applied problems. We have established a strong reputation for the development of techniques that involve the interfacing of separation methods - gas chromatography, liquid chromatography, and capillary zone electrophoresis - with mass spectrometry. Optimization of these sample delivery systems has enabled us to advance the state of the art for trace level analysis of biological samples. Much of our applied work is being done in collaboration with colleagues from the biomedical community in the Greater Boston area and beyond.

Selected Publications

• Lin Y, Schiavo S, Orjala J, Vouros P, Kautz R. Microscale LC-MS-NMR platform applied to the identification of active cyanobacterial metabolites. Anal Chem. 2008 80(21):8045-8054. (link)

  Wood JT, Williams JS, Pandarinathan L, Courville A, Keplinger MR, Janero DR, Vouros P, Makriyannis A, Lammi-Keefe CJ.  "Comprehensive profiling of the human circulating endocannabinoid metabolome: clinical sampling and sample storage parameters."  Clin Chem Lab Med. 2008 Jul 8. [Epub ahead of print]   pubmed
  

  Schiavo S, Ebbel E, Sharma S, Matson W, Kristal BS, Hersch S, Vouros P.  "Metabolite identification using a nanoelectrospray LC-EC-array-MS integrated system."  Anal Chem. 80(15):5912-23. (2008)  pubmed
  

  Nichols D, Lewis K, Orjala J, Mo S, Ortenberg R, O'Connor P, Zhao C, Vouros P, Kaeberlein T, Epstein SS.  "Short peptide induces an "uncultivable" microorganism to grow in vitro."  Appl Environ Microbiol. 74(15):4889-97. (2008)  pubmed
  

  Williams J, Wood J, Pandarinathan L, Karanian DA, Bahr BA, Vouros P, Makriyannis A. "Quantitative method for the profiling of the endocannabinoid metabolome by LC-atmospheric pressure chemical ionization-MS."  Anal Chem. ;79(15):5582-93. 2007 Aug 1; Epub 2007 Jun 29 pubmed 
  

• Xiong W, Glick J, Lin Y, Vouros P. "Separation and sequencing of isomeric oligonucleotide adducts using monolithic columns by ion-pair reversed-phase nano-HPLC coupled to ion trap mass spectrometry."  Anal Chem. 2007 Jul 15;79(14):5312-21. Epub 2007 Jun 19  pubmed 
  
• Black TM, Andrews CL, Kilili G, Ivan M, Tsichlis PN, Vouros P. Characterization of phosphorylation sites on Tpl2 using IMAC enrichment and a linear ion trap mass spectrometer. J Proteome Res. 2007 Jun;6(6):2269-76. Epub 2007 May 2 pubmed 
  
• Liao, Q., Chiu, N. H. L., Shen, C., and Vouros, P., “Investigation of the Enzymatic Behavior of Benzonase /Alkaline Phosphatase in the Digestion of DNA and DNA Adducts by ESI-LC/MS”, Anal. Chem., 79, 1907-1917 (2007). pubmed 
  
  
• Williams J, Pandarinathan L, Wood J, Vouros P, Makriyannis A. Endocannabinoid metabolomics: a novel liquid chromatography-mass spectrometry reagent for fatty acid analysis. AAPS J. 2006 Oct 20;8(4):E655-660. DOI: 10.1208/aapsj080474.   free full text
  
• Reddy GS, Robinson M, Wang G, Palmore GT, Gennaro L, Vouros P, De Clercq P, Vandewalle M, Young W, Ling S, Verstuyf A, Bouillon R. Removal of C-ring from the CD-ring skeleton of 1alpha,25-dihydroxyvitamin D3 does not alter its target tissue metabolism significantly. Arch Biochem Biophys. 2007 Apr 15;460(2):254-61. Epub 2006 Nov 21 pubmed
  
• Levin DS, Vouros P, Miller RA, Nazarov EG. Using a nanoelectrospray-differential mobility spectrometer-mass spectrometer system for the analysis of oligosaccharides with solvent selected control over ESI aggregate ion formation. J Am Soc Mass Spectrom. 2007 Mar;18(3):502-11. Epub 2006 Nov 30.  pubmed 
  
• Ricicki EM, Luo W, Fan W, Zhao LP, Zarbl H, Vouros P.  Quantification of N-(deoxyguanosin-8-yl)-4-aminobiphenyl adducts in human lymphoblastoid TK6 cells dosed with N-hydroxy-4-acetylaminobiphenyl and their relationship to mutation, toxicity, and gene expression profiling. Anal Chem. 2006 Sep 15;78(18):6422-32   pubmed
  
• Levin DS, Miller RA, Nazarov EG, Vouros P.  Rapid separation and quantitative analysis of peptides using a new nanoelectrospray- differential mobility spectrometer-mass spectrometer system. Anal Chem. 2006 Aug 1;78(15):5443-52  pubmed
  
• Andrews, C.L., Li, F., Yu, C.P., Yang, E., and Vouros, P., “Incorporation of a Nanosplitter Interface into an LC-RD-MS System to Facilitate Drug Metabolism Studies”, Journal of Mass Spectrom. 41, 43-49 (2006).  pubmed 
  
  
• Levin, D.S., Vouros, P., Miller, R.A., Nazarov, E.G., Morris, J.C., “Characterization of gas phase interactions utilizing a novel nanoelectrospray ionization - differential mobility - mass spectrometer (nanoESI-DMS-MS) system”, Anal. Chem., 78, 96-106 (2006).  pubmed 
  
• Argoti D, Liang L, Conteh A, Chen L, Bershas D, Yu CP, Vouros P, Yang E. Cyanide trapping of iminium ion reactive intermediates followed by detection and structure identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Chem Res Toxicol. 2005 Oct;18(10):1537-44.  pubmed 
  
• Ricicki, E.M., Andrews, C.L., Schiavo, S., and Vouros, P., “Analysis of DNA Adducts”, in Encyclopedia of Mass Spectrometry, Volume 3, Biological Applications Part B: Carbohydrates, Nucleic Acids and other Biological Compounds, Series editors: Caprioli, R. and Gross, M., Elsevier Ltd., Oxford, 2006, pp. 61-85.
  
• Simionato, A.V.C. , de Souza, G.D., Rodrigues-Filho,E., Glick, J., Vouros, P., and Carrilho, E., “Tandem mass spectrometry of coprogen and deferoxamine hydroxamic siderophores”, Rapid Commun. Mass Spectrom., 20, 193-199 (2006). pubmed 
  
• Flarakos J, Morand KL, Vouros P.  High-throughput solution-based medicinal library screening against human serum albumin. Anal Chem. 2005 Mar 1;77(5):1345-53.  pubmed 
  
• Andrews CL, Yu CP, Yang E, Vouros P. Improved liquid chromatography-mass spectrometry performance in quantitative analysis using a nanosplitter interface. J Chromatogr A. 2004 Oct 22;1053(1-2):151-9. pubmed 

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