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Barry L. Karger
Director,
James L. Waters Chair
in Analytical Chemistry
The Barnett Institute, 341 Mugar
Northeastern University
360 Huntington Avenue
Boston MA 02115
email: b.karger@neu.edu
voice: 617-373-2867
fax: 617-373-2855
Karger Research Group Page |
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Education
1960 B.S., Chemistry, MIT
1963 Ph.D. Chemistry,
Cornell University
Research Interests
- Trace
Level Comprehensive Characterization of Complex Proteins
- Use
of large peptide fragment analysis for high sequence coverage of phosphorylated,
glycosylated and other post-translationally modified structures.
- Analysis
of kinetics of phosphorylation upon stimulation by extra-cellular
signals of tyrosine kinase receptors.
- Determination
of binding partners of receptors upon stimulation by extra-cellular
signals, e.g. drugs, after immunoprecipitation.
- Analysis
of Complex Proteins Using a Combination of Collision Induced Dissociation
(CID) and Electron Transfer Dissociation (ETD).
- Trace
level analysis of phosphorylated proteins using CID and ETD
- Glycosylation
analysis using CID for structure and ETD for site localization
- Studies
of fragmentation of charge reduced species
- Ultra-Trace
Analysis of Proteomic Samples Using Very Narrow Bore LC Columns
- Monolithic
packed columns of polystyrene divinylbenzene with 20 micron i.d..
- Ultra-trace
analysis using 10 micron and lower porous layer open tubular (PLOT)
columns
- Development
of miniaturized on-line sample processing and analysis by LC/MS.
- Differential
Quantitative Analysis of Laser Capture Micro-Dissection (LCM) Samples
from Breast Cancer Tumors.
- Trace
analysis of LCM samples from breast cancer patients
- Analysis
of paraffin embedded formalin fixed tissue.
- New
Platform for Characterization of Disulfide Linkage and Glycoheterogeneity
at Specific Sites on Proteins
- Complete
disulfide mapping of biopharmaceuticals by ETD.
- Disulfide
linkages in immunopurified proteins O2 stress
- Glycan
analysis at the fmole to low pmole level of proteins.
- SRM analysis of glycoproteins.
- Discovery
and Implementation of Monoclonal Antibodies as Specific Reagents for
Biomarker Analysis for Use in Clinical Diagnosis
- Discovery
and generation of monoclonal antibodies to low-level disease specific
markers in plasma.
- Implementation
of protein microarray monoclonal antibodies for diagnostic analysis.
- Elucidation
of clinical relevance of discovery of disease specific biomarkers.
- Development
of Bioinformatic Tools for Proteomic Analysis
- Algorithms
for quantitative analysis of label-free and isotopically labeled samples
- Novel
tools for selection and annotation of CID spectra of glycopeptides
- De
novo peptide sequencing and identification
of PTMs
- Integration
of proteomic data with metabolic and regulatory pathways
- New
Methodologies for Proteomic Analysis
- Proteomic
analysis of organisms with unsequenced genomes
- Identification
of metabolic bottlenecks in the production of important valuable anti-cancer
compounds in plant cell cultures
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Awards (Partial Listing) |
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(1) |
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American Chemical Society Award in Chromatography, sponsored by Supelco,
Inc., Las Vegas, Nevada, 1982; |
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(2) |
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American Chemical Society Award in Analytical Chemistry sponsored
by the Fisher Scientific Company, 1990; |
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(3) |
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1998 ACS Award in Separations Science and Technology sponsored by
IBC Advanced Technologies, Inc. and Millipore Corporation, 15th ACS
National Meeting, Dallas, TX, 1998; |
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(4) |
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2002 Halasz Medal of the Hungarian Chemical Society for Chromatography
and Separation Science; |
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(5)
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2003
CaSSS Award for Distinguished Contributions to Separation Science |
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(6) |
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2004
Michael Widmer Award, sponsored by the New Swiss Chemical Society
and Novartis, Salzburg, Austria 2004. |
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(7)
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2003
CaSSS Award for Distinguished Contributions to Separation Science |
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(8)
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2008
Torbern Bergman Medal from the Swedish Chemical Society, Analytical
Division. |
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(9)
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2009
Marcel Golay Medal .(press
release) |
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(10) |
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Honorary
Symposium for Lifetime Achievements, at HPLC
2009 (Dresden, Germany)
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Recent Publications
Zang T, Dai S, Chen D, Lee BW, Liu S, Karger BL, Zhou ZS. Chemical
methods for the detection of protein N-homocysteinylation via selective
reactions with aldehydes. Anal Chem. 2009 Nov 1;81(21):9065-71.(pubmed)
Thakur D, Rejtar T, Karger BL, Washburn NJ, Bosques CJ, Gunay NS, Shriver
Z, Venkataraman G. Profiling the Glycoforms of the Intact
alpha Subunit of Recombinant Human Chorionic Gonadotropin by High-Resolution
Capillary Electrophoresis-Mass Spectrometry. Anal Chem. 2009
Nov 1;81(21):8900-7. (pubmed)
Jia Y, Wu SL, Isenberg JS, Dai S, Sipes JM, Field L, Zeng B, Bandle RW,
Ridnour LA, Wink DA, Ramchandran R, Karger BL, Roberts DD. Thiolutin
inhibits endothelial cell adhesion by perturbing Hsp27 interactions with
components of the actin and intermediate filament cytoskeleton. Cell
Stress Chaperones.2009 1355-8145. (link)
Monzo A, Rejtar T, Guttman A. Optimization of poly(GMA-co-EDMA)
Monolithic Support for Trypsin Nanoreactor Fabrication. J Chromatogr
Sci. Jul 2009; 47(6):467-72. (pubmed)
Karger BL, Guttman A. DNA sequencing by CE. Electrophoresis,
Jun 2009; 30 Suppl 1:S196-202.(pubmed)
Lazarev AV, Rejtar T, Dai S, Karger BL. Centrifugal methods and
devices for rapid in-gel digestion of proteins. Electrophoresis.
Mar 2009; 30(6):966-73. (pubmed)
Jiang H, Wu SL, Karger BL, Hancock WS. Mass spectrometric
analysis of innovator, counterfeit, and follow-on recombinant human growth
hormone. Biotechnol Prog. Jan-Feb 2009; 25(1):207-18. (pubmed)
Wu, Shiaw-Lin; Jiang, Haitao ; Lu, Qiaozhen ; Dai, Shujia ; Hancock,
William S. and Karger, Barry L. Mass Spectrometric Determination
of Disulfide Linkages in Recombinant Therapeutic Proteins Using Online
LC-MS with Electron-Transfer Dissociation Anal. Chem.
2009 81(1):112-22 (link)
Luo Q, Rejtar T, Wu SL, Karger BL Hydrophilic interaction 10mum
I.D. porous layer open tubular columns for ultratrace glycan analysis
by liquid chromatography-mass spectrometry. J Chromatogr A.,
Feb 2009;1216(8):1223-31(pubmed)
Dai S, Jia Y, Wu SL, Isenberg JS, Ridnour LA, Bandle RW, Wink DA, Roberts
DD, Karger BL. "Comprehensive Characterization of Heat Shock Protein
27 Phosphorylation in Human Endothelial Cells Stimulated by the Microbial
Dithiole Thiolutin." J Proteome Res. Aug 23 2008,
. 7(10):4384-95 pubmed
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