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Roger W. Giese
Senior Faculty Fellow,
Professor of Chemistry and Biomedical Science,
Director of the Environmental Cancer Research Program
The Barnett Institute, 341 Mugar
Northeastern University
360 Huntington Avenue
Boston MA 02115
email r.giese@neu.edu
voice: 617-373-3227
fax: 617-373-8720, -2855 |
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Dr. Giese's research is in the detection and identification of trace biomolecules such as genotoxins, DNA adducts, steroids, and bio-active peptides by mass spectrometry. The work combines analytical chemistry, organic chemistry, and biochemistry to advance the reagents and techniques available for sample preparation. Novel tags are developed for derivatization to increase analyte response and selectivity. A recent example is the development of a nucleotide exposure mass profile test which discovers genotoxins in environmental mixtures. In this test, a DNA nucleotide or strand is used as bait to catch genotoxic chemicals, giving specific changes in mass. Because accurate masses are determined, a connection can be made to corresponding DNA adducts in biological samples such as human blood lymphocytes. The opportunity to scale up environmental samples provides a pathway to structural characterization of unknown genotoxins. Below is a figure demonstrating the diversity of DNA adducts that can be detected simultaneously with high sensitivity and similar response with this technology.
Environmental Cancer Research Program Site
Prof. Giese's Research Group Site
Selected Publications (of 170 total)
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Yong,
L. C., Schulte, P. A., Kao, C-K., Giese, R. W., Boeniger,
M. F., Strauss, G. H.S., Petersen, M. R., Wiencke,
J. K., "DNA Adducts in Granulocytes of Hospital
Workers Exposed to Ethylene Oxide", Am. J.
Ind. Med., 50, 293-302.09 (2007).
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Shao, G., Kautz, R., Peng, S., Cui, G., Giese, R.
W., "Calibration by NMR for quantitative analysis:
p-Toluenesulfonic acid as a reference substance",
J. Chromatogr. A, 1138, 305-308 (2007).
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Murugaiah, V., Naim, A., Peng, S., Cui, G., Giese,
R. W., "4-(4'-Methyltetrafluorophenyl)-2,3,5,6-tetrafluorobenzyl
bromide: A new electrophoric derivatizing reagent",
J. Chromatogr113. A, 4, 338-340 (2006).
- Shimelis, O., Giese, R. W., "Nuclease P1 digestion/high-performance
liquid chromatography, a practical method
for DNA quantitation", J. Chromatogr.
A., 1117, 132-136 (2006).
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Kao, C-Y., Giese, R.W., Measurement of N7(2-Hydroxyethyl)guanine
in Human DNA by Gas Chromatography Electron Capture Mass
Spectrometry, Chem. Res. Toxicol, 18, 70-75 (2005).
- Pashkova,
A., Chen, H., Rejtar, T., Zang, X., Giese, R., Andreev,
V., Moskovets, E. Karger, Barry L. "Coumarin Tags for
Improved Analysis of Peptides by MALDI-TOF MS and MS/MS.
II. Alexa Fluor 350 Tag for Increased Peptide and Protein
Identification by LC-MALDI-TOF/TOF MS", Anal. Chem.,
4(1), 129-135 (2005).
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