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William S. Hancock
Faculty Fellow, Bradstreet Chair in Bioanalytical Chemistry
co-chair, trans-NIH Alliance of Glycobiologistsfor Detection of Cancer
and Cancer Risk
editor-in-chief, Journal of Proteome Research (ACS)
The Barnett Institute,
341 Mugar Hall
Northeastern University
360 Huntington Avenue
Boston, MA 02115
e-mail: wi.hancock@neu.edu
voice: 617-373-4881
fax: 617-373-2855
Hancock Research
Group Page
Chem.
Dept. Page |
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Education
1970 Ph.D., Adelaide University,
South Australia
1966 B.Sc., Adelaide University,
South Australia
Research Interests
The mission of Dr. Hancock's research program is the study of Glycoproteomics
and glycans associated with cancer and infectious disease. Our studies
involve the analysis of candidate biomarkers from both patient tissue
and blood samples and we use panels of lectins (sugar binding proteins)
to isolate and detect changes in glycan composition in disease. Current
active area include breast and renal cancer and study of the AIDS virus.
The second area of research is the study of biotechnology protein drugs
which is an area which requires that latest in mass spectrometry technology
especially with the emergence of biogeneric drugs. We are currently analyzing
growth hormone, interferon and antibody drugs and developing new analytical
approaches for study variants caused by changes in disulfides, glycosylation
as well as degradative reactions such as oxidation, deamidation and aggregation.
Recent
Invited Lectures: |
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(1) |
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6th Early Detection Research Network (EDRN) Scientific
Workshop and NCI Alliance of Glycobiologists Meeting, September 3rd,
Bethesda, MD, USA,"Glycan Markers for the Early Detection of
Cancer". |
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(2) |
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State of the Art Protein Analysis and Regulatory Science, August
31st to September 3rd, 2009, Boston, MA, "Modifications of Biopharmaceuticals".
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(3) |
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HPLC 2009, June 28th to July 2nd, 2009, Dresden,
Germany, "The Role of HPLC in the Discovery of Glycoprotein (Glycan)
Markers for the Early Detection of Cancer". |
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(4) |
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Clinical and Translational Research on Cancer: Glycomics Applications,
March 24th to 27th, Toba, Japan, "Glycoprotein
Markers for the Early Detecion of Cancer". |
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(5) |
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33rd International Symposium on HPLC,
Dec. 2 - 5, 2008, Kyoto, Japan, "The Use of Plasma Proteomics
to Study". |
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(6) |
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2008 Eastern Asian Glycoscience Symposium, Nov. 28-29, Daejeon, Korea, "
The Use of Plasma Glycoproteomics for the Early Detection of Disease".
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(7) |
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30th
European Peptide Symposium, Helsinki, Finland, Aug. 31st
to Sept. 5, "The Use of Plasma Proteomics to Study Disease Pathogenesis"th,
2008. |
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(8) |
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HUPO
7th World Congress, Aug. 16th to 20th
, 2008, Amsterdam, Netherlands, "The Use of Plasma Proteomics
to Study Disease Pathogenesis". |
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HPLC
2008, May 11th to 16th, 2008, Baltimore, MD,
"Signal Amplification in Clinical Proteomics". |
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Recent
Awards (Partial Listing):
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(1) |
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President,
US HUPO, 2008 |
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(2) |
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Randolph
T. Major Memorial Lecturer (R.T.Major Lecture Series, Dept. of Chem.,
Univ. of Connecticut, 2004) |
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(3) |
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ACS
Award in Separation Science (ACS
National Meeting, 2003) |
| Publications:
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206
scientific publications, plus 7 books
and 21 patents. |
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Complete
List of Publications
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Current Pubmed search "W.S.
Hancock"
Selected Recent
Publications: |
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Zeng, Z., Hincapie, M., Haab, B.B., Hanash, S., Pitteri, S.J., Kluck,
S., Hogan, J.M., Kennedy, J. and Hancock, W.S.,"The Development
of an Integrated Platform to Identify Breast Cancer Glycoproteome
Changes in Human Serum", J Chromatogr A., (2009) Sep 16. [Epub
ahead of print]. (pubmed)
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| (2) |
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Lu, Q., Zheng, X., McIntosh, T., Davis, H., Nemeth, J.F., Pendley,
C., Wu, S.-L and Hancock W.S., "Development of Different Analysis
Platforms with LC-MS for Pharmacokinetic Studies of Protein Drugs",
Anal. Chem. (2009) Nov 1;81(21):8715-23. (pubmed)
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Akella, L.M., Rejtar, T., Orazine, C., Hincapie, M. and Hancock, W.S.,
"CLUE-TIPS, Clustering Methods for Pattern Analysis of LC-MS
Data", J Proteome Res. (2009) Oct;8(10):4732-42. (pubmed)
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Taylor,
A.D., Hancock, W.S., Hincapie, M., Taniguchi, N. and Hanash, S.M.,
"Towards an Integrated Proteomic and Glycomic Approach to Finding
Cancer Biomarkers", Genome Med., Jun 4, 1(6):57, (2009). (pubmed)
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| (5) |
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Bell,
A.W., et al,"A HUPO Test Sample Study Reveals Common Problems in Mass
Spectrometry-Based Proteomics", Nat Methods, (2009) 6(6), 423-30.
(pubmed)
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Jiang, H., Wu, S-L., Karger,B.L. and Hancock, W.S.,
"Mass Spectrometric Analysis of Innovator, Counterfeit, and Follow-On
Recombinant Human Growth Hormone", Biotechnol. Prog., Jan.-Feb.,
25(1), 207-18 (2009). (pubmed)
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| (7) |
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Zheng, X., Wu, S-L., Hincapie, M. and Hancock, W.S.,"Study of the
Human Plasma Proteome of Rheumatoid Arthritis", J. Chromatogr.
A, 1216(16), 3538-45 (2009). (pubmed)
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Wu, S-L., Jiang, H., Lu, Q., Dai, S., Hancock, W.S. and Karger, B.L.,
"Mass Spectrometric Determination of Disulfide Linkages in Recombinant
Therapeutic Proteins Using Online LC-MS with Electron-Transfer Dissociation",
Anal. Chem., 81, 1, 112-122 (2009).(link) |
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| (9) |
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Ochoa, C.D., Baker, H., Hasak, S., Matyal, R., Salam, A., Hales, C.A.,
Hancock, W.S. and Quinn, D.A., "Cyclic Stretch Affects Pulmonary Endothelial
Cell Control of Pulmonary Smooth Muscle Cell Growth", Am. J. Respir.
Cell Mol. Biol. 1, 105-112 (2008). (pubmed) |
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Pandey, A., et. al., (Hancock W.S.) "Human Proteinpedia enables sharing
of human protein data", Nat Biotechnol. , Feb;26(2):164-7 (2008).(pubmed)
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Kullolli,
M., Hancock, W.S., Hincapie, M., "Preparation of a high-performance
multi-lectin affinity chromatography (HP-M-LAC) adsorbent for the
analysis of human plasma glycoproteins", J, Sep Sci. (14):2733-9
(2008).(pubmed)
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| (12) |
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Lu,
C., Murugesan, N.. Macdonald, J. A, Wu, S. L., Pachter, J. and
Hancock, W. S., "Analysis of Mouse Brain Microvascular Endothelium
Using Immuno-Laser Capture Microdissection Coupled to a Hybrid LTQ-FT
MS Proteomics Platform”, Electrophoresis, 2689-95 (2008).(pubmed) |
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| (13) |
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Ralin,
D.W., Dultz, S.C., Silver, J.E., Kullolli, M., Hancock, W.S. and Hincapie,
M., "Kinetic Analysis of Glycoprotein-lectin Interactions by
Label- Free Internal Reflection”, Clin. Proteomics, 4,37-46.
(2008).(link) |
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| (14) |
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Plavina, T., Hincapie, M., Wakshull, E., Subramanyam M., and Hancock,
W.S., “Increased Levels of Cytoskeletal and Ca2+-Binding Proteins
and their Peptides in Plasma of Psoriasis Patients”, Clin. Chem.,
54,1805-1814 (2008).(pubmed) |
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| (15) |
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Dayarathna, M.K., Hancock, W.S. and Hincapie, M., "A Two-Step
Fractionation Approach for Plasma Proteomics Using Immunodepletion
of Abundant Proteins and Multi-lectin Affinity Chromatography (M-LAC):
Application to the Analysis of Obesity, Diabetes and Hypertension,",
J. Sep. Science, 1156-1166 (2008).(pubmed) |
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Orazine, C. I., Hincapie, M., Hancock, W.S., Hattersley, M., and Hanke,
J., "A Proteomic Analysis of the Plasma Glycoproteins of a MCF-7
Mouse Xenograft : A Model System for the Detection of Tumor Markers",
J. Prot. Res., 7, 1542-54 (2008). (pubmed) |
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Wu, S.-L., Gu, Y., Hanlon, D., Meyer, J., Linder, J., Hancock, W.S.,
and Karger, B.L., "Proteomic Analysis of High-Grade Dysplastic
Cervical Cells Obtained From ThinPrep® Slides Using Laser Capture
Microdissection and Mass Spectrometry", J. Proteome Res.,
6, 4256-68 (2007). |
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| (18) |
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Plavina, T., Wakeshull, E., Hancock, W.S., Hincapie, M., "Combination
of abundant protein depletion and multi-lectin affinity chromatography
(M-LAC) for plasma protein biomarker discovery", J. Proteome
Res., 662-671 (2007). |
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Zheng, X., Baker, H., and Hancock, W. S., "Analysis of the Low
Molecular Weight Serum Peptidome Using Ultrafiltration and a Hybrid
Ion Trap-Fourier Transform Mass Spectrometer", J. Chromatogr.,
1120, 173-184 (2006). |
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Plymoth, A., Yang, Z., Lofdahl, C. G., Ekberg-Jansson, A., Dahlback,
M., Fehniger, T. E., Marko-Varga, G., Hancock, W. S., "Rapid
Proteome Analysis of Bronchoalveolar Lavage Samples of Lifelong Smokers
and Never-Smokers by Micro-Scale Liquid Chromatography and Mass Spectrometry",
Clin. Chem., 52, 671-679 (2006). |
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| (21) |
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Wang, Y., Wu, S- L. and Hancock, W. S., "Approaches to the Study
of N-linked Glycoproteins in Human Plasma Using Lectin Affinity Chromatography
and Nano-HPLC Coupled to Electrospray Linear Ion Trap-Fourier Transform
Mass Spectrometry (LTQ/FTMS)", Glycobiology, 16, 514-523
(2006). |
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Wang, Y., Wu, S- L. and Hancock, W. S., "Monitoring of Glycoprotein
Products in Cell Culture Lysates Using Lectin Affinity Chromatography
and Capillary-HPLC Coupled to Electrospray Linear Ion Trap - Fourier
Transform Mass Spectrometry LTQ/FTMS)", Biotechnology Progress,
22, 873-880 (2006). |
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| (23) |
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Zheng, X., Wu, S- L., and Hancock, W. S., "Glycation of Interferon-beta-1b
and human serum albumin in a lyophilized glucose formulation",
Int. J. Pharmaceutics, 322, 136-145 (2006). |
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| (24) |
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Yang, Z., Harris, L. E., Palmer-Toy, D. E. and Hancock, W. S., "Characterization
of Multiple Glycoprotein Biomarker Candidates in Serum from Breast
Cancer Patients Using Multi-lectin Affinity Chromatography (M-LAC)",
Clin. Chem., 52, 1897-1905 (2006). |
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Wang, Y., Dai, D., Sadee, W. and Hancock, W.S., "A Pharmacoproteomics
Study of the Cancer Cell Line EKVX", Mol. Pharmaceutics,
5, 566-578 (2006). |
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Zheng, X., Baker, H., Hancock, W. S., Fawaz, F., McCaman, M. and Pungor,
E., "Proteomic Analysis for the Assessment of Different Lots
of Fetal Bovine Serum as a Raw Material for Cell Culture", Biotechnology
Progress, 5, 1294-1300 (2006). |
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updated: 11/03/09
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