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Other Recent Projects of the Karger Laboratory
LC-MALDI-MS:
Multidimensional LC-MS .
Proteome Analysis of Methanosarcina acetivorans
LC-MALDI-MS:
Multidimensional LC-MS
The
integration of LC with MALDI-TOF MS has been advanced in several
ways. Improved data processing of LC-MS 2D spectra can
remove chemical noise, greatly enhancing signal-to-noise ratios
of low abundance components.
Combined with algorithms for optimizing a precursor ion list
for MS/MS analysis, we can identify over 20% more unique proteins
than using standard data processing approaches.
To increase the throughput of MALDI-TOF MS, we have built
a MALDI spectrometer utilizing a laser with a 2 kHz repetition-rate
laser, over 10-fold faster than commercial (10-300 Hz) instruments.
Its data acquisition rate of 2 samples per second cuts
the MS analysis time from 4.5 hours to 12 minutes, per plate of
700 samples. When used with
a system built to multiplex multiple LC separations into
parallel depositions on a MALDI plate, fast MALDI significantly
increases LC-MS throughput relative to on-line (ESI) methods.
Coupling
of High-Resolution CE and LC to MALDI-MS
Overview by Tomas
Rejtar
- High-speed,
high-resolution monolithic capillary LC-MALDI MS using an off-line
continuous deposition interface for proteomic analysis.
Paper
(Anal Chem 2005)
- A New Strategy
for Comprehensive Proteome Analysis Using Parallel Replicate Separation
with Multiplexed LC-MALDI MS Poster
Abstract (Chen ASMS 2004)
- Universal
Deposition Device for Off-line Coupling of LC to MALDI MS and
MS/MS Poster
Abstract (Rejtar ASMS 2004)
- Closely
Spaced External Standards: a Universal Method of Achieving 5 ppm
Mass Accuracy Over the Entire MALDI Plate in Axial MALDI-TOF MS
Paper
(Moskovets RCMS 2003) Poster
(ASMS '02)
- Comprehensive
Proteome Analysis by Multi-Dimensional Separation Coupled to High
Mass Accuracy MALDI-MS and MALDI-MS/MS Poster
Abstract (Chen, ASMS 2003)
High-Throughput
2 kHz LC-MALDI-TOF Spectrometer Summary
by Eugene Moskovets
- Rapid Quantitative
Analysis of Selected Serum Glycoproteins Using Isotopically Labeled
Peptides By 2 kHz LC -MALDI MS Full
Poster
- MALDI TOF-MS
System with High-Repetition Rate Laser for Fast Analysis of Multiplexed
Separated Peptide Mixtures Poster
Abstract (Moskovets ASMS 2004)
- MALDI-TOF
MS with 2-KHz Laser for Fast Analysis of Separated Peptide Digests
Poster Abstract
(Moskovets ASMS 2003)
Proteome
Analysis of the archeabacterium Methanocarcina acetivorans C2A
Overview (Lingyun Li)
High-throughput
Microcoil NMR of Compound Libraries Using Zero-Dispersion Segmented
Flow Analysis Paper
(Kautz, J. Comb. Chem., 2005)
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