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William S. Hancock
Education
1970 Ph.D., Adelaide University,
South Australia
1966 B.Sc., Adelaide University,
South Australia
Research Interests
We have formed a tumor glycome laboratory team with multi-disciplinary
expertise in glycomics, proteomics, informatics, and cancer markers to
apply an integrated glycoproteomics approach to discover glycan biomarkers
for early detection of cancer. One major goal is focused on the analysis
and identification of glycoproteins in plasma and tissue to detect differences
between breast cancer subjects and controls. We are developing analytical
approaches to identify and characterize the glycan changes associated
with these glycoproteins. At present we have combined the multi-lectin
platform (M-LAC) with 1D-SDS PAGE, lectin blotting and proteomic analysis
as well as deeper fractionation on the new Protein Forest IEF chip. For
glycoproteins of interest, the number of glycosylation sites, the attachment
point of the glycan to the polypeptide backbone and the accurate mass
and fragmentation pattern of individual glycopeptides in the LT-FTMS and
LTMS with both CID and ETD will be determined. Additionally glycans will
be cleaved and their structure determined after permethylation by MALDI-TOF
analysis of the resulting glycan HPLC profiles. Candidate glycoprotein
markers resulting from these studies will be prioritized based on elevated
levels in both breast tumor tissue and plasma and occurrence of altered
glycosylation. In addition, we are studying the glycan structures of the
secreted form of EGFR from A431 cell lines as a model system for understanding
potential glycosylation differences between secreted and plasma membrane
forms of cancer associated signaling molecules. We plan to achieve the
translational aspect of this study by the application of a high throughput
and sensitive glycan assay platform which will be used to pre-validate
markers in a large sample set and thus facilitate subsequent clinical
studies. Currently we are investigating the application of the antibody-lectin
array approach in collaboration with the Haab laboratory and a targeted
MRM approach.
Recent
Invited Lectures: |
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(1) |
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6th Early Detection Research Network (EDRN) Scientific
Workshop and NCI Alliance of Glycobiologists Meeting, September 3rd,
Bethesda, MD, USA,"Glycan Markers for the Early Detection of
Cancer". |
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(2) |
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State of the Art Protein Analysis and Regulatory Science, August
31st to September 3rd, 2009, Boston, MA, "Modifications of Biopharmaceuticals".
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(3) |
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HPLC 2009, June 28th to July 2nd, 2009, Dresden,
Germany, "The Role of HPLC in the Discovery of Glycoprotein (Glycan)
Markers for the Early Detection of Cancer". |
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(4) |
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Clinical and Translational Research on Cancer: Glycomics Applications,
March 24th to 27th, Toba, Japan, "Glycoprotein
Markers for the Early Detecion of Cancer". |
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Recent Awards (Partial Listing):
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(1) |
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President, US HUPO, 2008 |
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(2) |
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Randolph T. Major Memorial Lecturer (R.T.Major Lecture Series, Dept. of Chem.,
Univ. of Connecticut, 2004) |
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(3) |
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ACS
Award in Separation Science (ACS
National Meeting, 2003) |
| Publications: |
206 scientific publications, plus 7 books
and 21 patents. |
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Complete
List of Publications
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Current Pubmed search "W.S.
Hancock"
Selected Recent Publications: |
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Zeng, Z., Hincapie, M., Haab, B.B., Hanash,
S., Pitteri, S.J., Kluck, S., Hogan, J.M., Kennedy, J. and Hancock,
W.S.,"The Development of an Integrated Platform to Identify Breast
Cancer Glycoproteome Changes in Human Serum", J Chromatogr A.,
(2009) Sep 16. [Epub ahead of print]. (pubmed)
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Lu, Q., Zheng, X., McIntosh, T., Davis,
H., Nemeth, J.F., Pendley, C., Wu, S.-L and Hancock W.S., "Development
of Different Analysis Platforms with LC-MS for Pharmacokinetic Studies
of Protein Drugs", Anal. Chem. (2009) Nov 1;81(21):8715-23. (pubmed)
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Akella, L.M., Rejtar, T., Orazine, C.,
Hincapie, M. and Hancock, W.S., "CLUE-TIPS, Clustering Methods
for Pattern Analysis of LC-MS Data", J Proteome Res. (2009) Oct;8(10):4732-42.
(pubmed)
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Taylor, A.D., Hancock, W.S., Hincapie,
M., Taniguchi, N. and Hanash, S.M., "Towards an Integrated Proteomic
and Glycomic Approach to Finding Cancer Biomarkers", Genome Med.,
Jun 4, 1(6):57, (2009). (pubmed)
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Bell, A.W., et al,"A HUPO Test
Sample Study Reveals Common Problems in Mass Spectrometry-Based Proteomics",
Nat Methods, (2009) 6(6), 423-30. (pubmed)
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Jiang, H., Wu, S-L., Karger,B.L. and Hancock,
W.S., "Mass Spectrometric Analysis of Innovator, Counterfeit, and
Follow-On Recombinant Human Growth Hormone", Biotechnol. Prog.,
Jan.-Feb., 25(1), 207-18 (2009). (pubmed)
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Zheng, X., Wu, S-L., Hincapie, M. and Hancock,
W.S.,"Study of the Human Plasma Proteome of Rheumatoid Arthritis",
J. Chromatogr. A, 1216(16), 3538-45 (2009). (pubmed)
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Wu, S-L., Jiang, H., Lu, Q., Dai, S.,
Hancock, W.S. and Karger, B.L., "Mass Spectrometric Determination
of Disulfide Linkages in Recombinant Therapeutic Proteins Using Online
LC-MS with Electron-Transfer Dissociation", Anal. Chem., 81,
1, 112-122 (2009).(link)
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Ochoa, C.D., Baker, H., Hasak, S., Matyal,
R., Salam, A., Hales, C.A., Hancock, W.S. and Quinn, D.A., "Cyclic
Stretch Affects Pulmonary Endothelial Cell Control of Pulmonary Smooth
Muscle Cell Growth", Am. J. Respir. Cell Mol. Biol. 1, 105-112
(2008). (pubmed)
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Pandey, A., et. al., (Hancock W.S.) "Human
Proteinpedia enables sharing of human protein data", Nat Biotechnol.
, Feb;26(2):164-7 (2008).(pubmed)
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Kullolli, M., Hancock, W.S., Hincapie,
M., "Preparation of a high-performance multi-lectin affinity
chromatography (HP-M-LAC) adsorbent for the analysis of human plasma
glycoproteins", J, Sep Sci. (14):2733-9 (2008).(pubmed)
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Lu, C., Murugesan, N.. Macdonald, J. A,
Wu, S. L., Pachter, J. and Hancock, W. S., "Analysis
of Mouse Brain Microvascular Endothelium Using Immuno-Laser Capture
Microdissection Coupled to a Hybrid LTQ-FT MS Proteomics Platform”,
Electrophoresis, 2689-95 (2008).(pubmed) |
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Ralin, D.W., Dultz, S.C., Silver, J.E.,
Kullolli, M., Hancock, W.S. and Hincapie, M., "Kinetic Analysis
of Glycoprotein-lectin Interactions by Label- Free Internal Reflection”,
Clin. Proteomics, 4,37-46. (2008).(link) |
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| (14) |
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Plavina, T., Hincapie, M., Wakshull, E.,
Subramanyam M., and Hancock, W.S., “Increased Levels of Cytoskeletal
and Ca2+-Binding Proteins and their Peptides in Plasma of Psoriasis
Patients”, Clin. Chem., 54,1805-1814 (2008).(pubmed) |
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Dayarathna, M.K., Hancock, W.S. and Hincapie,
M., "A Two-Step Fractionation Approach for Plasma Proteomics
Using Immunodepletion of Abundant Proteins and Multi-lectin Affinity
Chromatography (M-LAC): Application to the Analysis of Obesity, Diabetes
and Hypertension,", J. Sep. Science, 1156-1166 (2008).(pubmed) |
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| (16) |
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Orazine, C. I., Hincapie, M., Hancock,
W.S., Hattersley, M., and Hanke, J., "A Proteomic Analysis of
the Plasma Glycoproteins of a MCF-7 Mouse Xenograft : A Model System
for the Detection of Tumor Markers", J. Prot. Res., 7,
1542-54 (2008). (pubmed)
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